1KD2
Crystal Structure of Human Deoxyhemoglobin in Absence of Any Anions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 299 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-08-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.38 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 97.339, 99.348, 66.123 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.870 |
| Rwork | 0.198 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hhb |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.613 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.007 | 1.910 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Number of reflections | 51030 | |
| Completeness [%] | 95.6 | 92.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH METHOD | 7 | 298 | DEIONIZED PEG 3350 12.5%, pH 7.0, BATCH METHOD, temperature 298K |
