1KCW
X-RAY CRYSTAL STRUCTURE OF HUMAN CERULOPLASMIN AT 3.0 ANGSTROMS
Experimental procedure
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 276 |
Detector technology | IMAGE PLATE |
Collection date | 1995-10 |
Detector | MARRESEARCH |
Wavelength(s) | 0.87, 0.882 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 213.920, 213.920, 85.630 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 12.000 - 3.000 |
R-factor | 0.22 |
Rwork | 0.220 |
R-free | 0.28600 |
Structure solution method | MIR |
RMSD bond length | 0.012 |
RMSD bond angle | 25.940 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((ROTAVATA)) |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.640 | 3.100 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.072 | 0.276 |
Number of reflections | 44533 | |
<I/σ(I)> | 7.4 | 2.7 |
Completeness [%] | 98.5 | 93.6 |
Redundancy | 7 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 5.45 * | 277 * | pH 5.65 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | hCP | 28-30 (mg/ml) | |
2 | 1 | drop | PEG20000 | 3 (%(w/v)) | |
3 | 1 | drop | 250 (mM) | ||
4 | 1 | drop | sodium acetate | 100 (mM) |