1KCT
ALPHA1-ANTITRYPSIN
Experimental procedure
Temperature [K] | 295 |
Detector technology | DIFFRACTOMETER |
Collection date | 1994-11-10 |
Detector | ENRAF-NONIUS FAST |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 114.870, 39.980, 92.420 |
Unit cell angles | 90.00, 102.82, 90.00 |
Refinement procedure
Resolution | 8.000 - 3.460 |
R-factor | 0.184 |
Rwork | 0.184 |
RMSD bond length | 0.015 |
RMSD bond angle | 26.420 * |
Data reduction software | MADNES |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 3.600 | |
High resolution limit [Å] | 3.460 | |
Rmerge | 0.106 | |
Number of reflections | 4380 | |
Completeness [%] | 82.6 | 63 |
Redundancy | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.9 * | pH 8.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | inhibitor | 10 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 50 (mM) | |
3 | 1 | reservoir | PEG4000 | 28.5 (%(w/v)) | |
4 | 1 | reservoir | Tris-HCl | 100 (mM) | |
5 | 1 | reservoir | lithium sulfate | 200 (mM) |