1KCM
Crystal Structure of Mouse PITP Alpha Void of Bound Phospholipid at 2.0 Angstroms Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-12-09 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.9322 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 50.464, 50.464, 216.105 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.216 * |
| Rwork | 0.216 |
| R-free | 0.27300 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1FVZ |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.420 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.060 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.051 | 0.171 |
| Total number of observations | 231585 * | |
| Number of reflections | 22060 | 2012 * |
| <I/σ(I)> | 25.7 | 7.3 |
| Completeness [%] | 96.7 | 91.1 |
| Redundancy | 4.33 * | 2.70 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 * | 4 * | 11% PEG 6000, 0.2M calcium acetate, 100MM cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 14 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | pH7.2 |
| 3 | 1 | drop | beta-mercaptoethanol | 10 (mM) | |
| 4 | 1 | reservoir | PEG6000 | 11 (%(w/v)) | |
| 5 | 1 | reservoir | calcium acetate | 200 (mM) | |
| 6 | 1 | reservoir | cacodylate | 100 (mM) | pH6.5 |
