1KCL
Bacillus ciruclans strain 251 Cyclodextrin glycosyl transferase mutant G179L
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-09-01 |
| Detector | MACSCIENCE |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 114.714, 109.473, 65.762 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.500 - 1.940 |
| R-factor | 0.152 * |
| Rwork | 0.171 |
| R-free | 0.18600 * |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1d3c |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.280 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 * | 1.990 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.116 | 0.372 |
| Number of reflections | 60480 | |
| <I/σ(I)> | 11.5 | 3.2 |
| Completeness [%] | 97.5 | 96.6 |
| Redundancy | 4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.55 * | 295 | Lawson, C.L., (1994) J.Mol.Biol., 236, 590. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MPD | 60 (%(v/v)) | |
| 2 | 1 | reservoir | HEPES | 100 (mM) | pH7.55 |
| 3 | 1 | drop | alpha-cyclodextrin | 10-20 (mg/ml) |
