1KCK
Bacillus circulans strain 251 Cyclodextrin glycosyl transferase mutant N193G
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ELLIOTT GX-21 |
Temperature [K] | 295 |
Detector technology | IMAGE PLATE |
Collection date | 1995-01-01 |
Detector | MACSCIENCE |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 120.686, 111.162, 65.944 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.640 - 2.430 |
R-factor | 0.153 * |
Rwork | 0.159 |
R-free | 0.19900 * |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2cxg |
RMSD bond length | 0.006 * |
RMSD bond angle | 1.250 * |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 * | |
High resolution limit [Å] | 2.430 | |
Number of reflections | 31710 | |
Completeness [%] | 92.1 * | 70.2 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.55 * | 295 | Lawson, C.L., (1994) J.Mol.Biol., 236, 590. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | MPD | 60 (%(v/v)) | |
2 | 1 | reservoir | HEPES | 100 (mM) | pH7.55 |
3 | 1 | drop | alpha-cyclodextrin | 10-20 (mg/ml) |