1KCG
NKG2D in complex with ULBP3
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9B |
Synchrotron site | NSLS |
Beamline | X9B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-05-21 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9639, 0.9792, 0.9795 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 62.050, 62.050, 237.310 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.000 - 2.600 |
R-factor | 0.22 * |
Rwork | 0.220 |
R-free | 0.26800 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.890 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.000 | 2.590 |
High resolution limit [Å] | 2.600 | 2.600 * |
Rmerge | 0.083 * | 0.400 * |
Number of reflections | 26678 * | 2683 * |
Completeness [%] | 99.7 * | 99.9 * |
Redundancy | 5.4 * | 5.2 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 297 | PEG 8000, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8-15 (mg/ml) | |
2 | 1 | reservoir | PEG3350-8000 | 10 (%) | |
3 | 1 | reservoir | MES | 50 (mM) | pH6.0 |