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1KCG

NKG2D in complex with ULBP3

Experimental procedure
Experimental methodMAD
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X9B
Synchrotron siteNSLS
BeamlineX9B
Temperature [K]100
Detector technologyCCD
Collection date2001-05-21
DetectorADSC QUANTUM 4
Wavelength(s)0.9639, 0.9792, 0.9795
Spacegroup nameP 43 21 2
Unit cell lengths62.050, 62.050, 237.310
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution41.000 - 2.600
R-factor0.22

*

Rwork0.220
R-free0.26800
Structure solution methodMAD
RMSD bond length0.009
RMSD bond angle1.890
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwareSOLVE
Refinement softwareCNS (1.0)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]41.0002.590
High resolution limit [Å]2.6002.600

*

Rmerge0.083

*

0.400

*

Number of reflections26678

*

2683

*

Completeness [%]99.7

*

99.9

*

Redundancy5.4

*

5.2

*

Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP6297PEG 8000, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 297K
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein8-15 (mg/ml)
21reservoirPEG3350-800010 (%)
31reservoirMES50 (mM)pH6.0

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