1KCF
Crystal Structure of the Yeast Mitochondrial Holliday Junction Resolvase, Ydc2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-03-31 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.93400 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.000, 133.940, 73.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.340 - 2.300 |
| R-factor | 0.238 * |
| Rwork | 0.238 |
| R-free | 0.27000 |
| Structure solution method | Single Wavelength Anomalous Dispersion |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.000 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.340 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.034 * | 0.219 * |
| Number of reflections | 31239 | |
| <I/σ(I)> | 16 | 3.6 |
| Completeness [%] | 91.9 | 90.5 |
| Redundancy | 3.4 * | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 4.6 | 287 | Ammonium Sulphate, Sodium acetate, pH 4.6, microbatch, temperature 287K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | ammonium sulfate | 2.0 (M) | or dihydrogen phosphate |
| 2 | 1 | 1 | sodium acetate | 0.1 (M) | pH4.6 |
| 3 | 1 | 1 | Tris-HCl | 0.1 (M) | pH8.5 |
| 4 | 1 | 1 | lithium sulfate monohydrate | 0.5 (M) | |
| 5 | 1 | 1 | PEG8000 | 15 (%(w/v)) |
