1KCA

Crystal Structure of the lambda Repressor C-terminal Domain Octamer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	AREA DETECTOR
Detector	MARRESEARCH
Wavelength(s)	1.54
Spacegroup name	C 1 2 1
Unit cell lengths	150.090, 113.720, 95.730
Unit cell angles	90.00, 110.26, 90.00

Refinement procedure

Resolution	23.530 - 2.910
R-factor	0.227 *
Rwork	0.227
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1f39
RMSD bond length	0.007
RMSD bond angle	1.500
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.500
High resolution limit [Å]	2.900	2.900
Rmerge	0.144	0.449
Total number of observations	53445 *
Number of reflections	33013
<I/σ(I)>	4.5	1.8
Completeness [%]	99.2	95
Redundancy	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	22 *	sodium formate, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	sodium formate	1.69 (M)
3	1	reservoir	sodium acetate	0.05 (M)	pH4.6