1KBW
CRYSTAL STRUCTURE OF THE SOLUBLE DOMAIN OF ANIA FROM NEISSERIA GONORRHOEAE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 100 |
Detector technology | DIFFRACTOMETER |
Collection date | 1999-05-15 |
Detector | WEISSENBERG |
Wavelength(s) | 1 |
Spacegroup name | P 1 |
Unit cell lengths | 66.230, 99.550, 103.710 |
Unit cell angles | 83.55, 73.70, 73.01 |
Refinement procedure
Resolution | 50.000 - 2.400 |
R-factor | 0.211 |
Rwork | 0.211 |
R-free | 0.23400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 * |
RMSD bond angle | 2.262 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.550 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.073 | 0.223 * |
Number of reflections | 76914 | 11789 * |
<I/σ(I)> | 6.96 | 2.5 |
Completeness [%] | 80.7 | 74 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 22 * | 1.7 M (NH4)2SO4, 0.2 M LiSO4 and 0.1 M CAPS, pH 10.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 1.7 (M) | |
2 | 1 | reservoir | 0.2 (M) | ||
3 | 1 | reservoir | CAPS | 0.1 (M) | pH10.5 |
4 | 1 | drop | protein | 15 (mg/ml) | |
5 | 1 | drop | Tris | 10 (mM) | pH7.0 |