1KBU
CRE RECOMBINASE BOUND TO A LOXP HOLLIDAY JUNCTION
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-02-27 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 107.170, 121.600, 179.310 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 5.000 - 2.200 |
R-factor | 0.231 |
Rwork | 0.231 |
R-free | 0.27900 |
Structure solution method | ISOMORPHOUS MOLECULAR REPLACEMENT |
Starting model (for MR) | combination of 1CRX and 4CRX (see publication for details) |
RMSD bond length | 0.006 |
RMSD bond angle | 1.610 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | TNT |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.500 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.033 * | 0.287 * |
Total number of observations | 161433 * | |
Number of reflections | 44903 * | |
<I/σ(I)> | 9.6 | 2.4 |
Completeness [%] | 89.0 | 89.8 |
Redundancy | 3.6 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7 * | 21-25 * | MPD, sodium acetate, calcium chloride, pH 5.00, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MPD | ||
2 | 1 | 1 | sodium acetate | ||
3 | 1 | 1 | CaCl2 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 35-45 (mg/ml) | |
2 | 1 | reservoir | MPD | 17-30 (%) | |
3 | 1 | reservoir | sodium acetate | 12.5-100 (mM) | pH5.0-5.5 |