1KBP

KIDNEY BEAN PURPLE ACID PHOSPHATASE

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1995-04-15
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	132.700, 347.300, 128.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.650
R-factor	0.196 *
Rwork	0.195
R-free	0.22300 *
RMSD bond length	0.021
RMSD bond angle	2.600 *
Data reduction software	DENZO
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
High resolution limit [Å]	2.650 *
Rmerge	0.066
Total number of observations	312508 *
Number of reflections	79375
Completeness [%]	93.0 *
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	12 (mg/ml)
2	1	drop	PEG10000	4 (%)
3	1	drop	sodium acetate	50 (mM)
4	1	reservoir	precipitant	15 (%)