1KAZ

70KD HEAT SHOCK COGNATE PROTEIN ATPASE DOMAIN, K71E MUTANT

Experimental procedure

Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS II
Spacegroup name	P 21 21 21
Unit cell lengths	143.500, 63.800, 46.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.700
R-factor	0.202
Rwork	0.202
R-free	0.23400
RMSD bond length	0.007
RMSD bond angle	23.860 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
High resolution limit [Å]	1.700 *
Rmerge	0.041
Total number of observations	134447 *
Number of reflections	42307
Completeness [%]	89.4
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	V8-venerated fragment	8 (mg/ml)
2	1	drop		1 (mM)
3	1	drop	ADP	1 (mM)
4	1	drop		1 (M)
5	1	reservoir	PEG8000	20 (%)