1KAM
Structure of Bacillus subtilis Nicotinic Acid Mononucleotide Adenylyl Transferase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2001-07-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9792, 0.9567 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.977, 126.103, 70.579 |
| Unit cell angles | 90.00, 92.73, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| Rwork | 0.222 |
| R-free | 0.25440 * |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.347 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.079 * | 0.470 |
| Total number of observations | 196259 * | |
| Number of reflections | 51680 * | |
| <I/σ(I)> | 2.4 | |
| Completeness [%] | 99.9 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 18 * | PEG3350, Magnesium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP at 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 14 (mg/ml) | |
| 2 | 1 | drop | HEPES | 50 (mM) | pH7.5 |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | reservoir | PEG3350 | 8 (%) | |
| 5 | 1 | reservoir | 100 (mM) |
