1KAF
DNA Binding Domain Of The Phage T4 Transcription Factor MotA (AA105-211)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-10-19 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9795, 0.9789, 0.9636 |
Spacegroup name | P 1 |
Unit cell lengths | 31.284, 54.733, 91.445 |
Unit cell angles | 94.77, 91.10, 95.61 |
Refinement procedure
Resolution | 30.000 - 1.600 |
R-factor | 0.226 * |
Rwork | 0.226 |
R-free | 0.25800 |
Structure solution method | MAD |
RMSD bond length | 0.007 * |
RMSD bond angle | 1.310 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.100 * | |
Total number of observations | 638591 * | |
Number of reflections | 87713 * | |
Completeness [%] | 92.0 | 83 * |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 5 * | 298 | from 19% PEG 8000, 35 mM potassium phosphate pH 5.0, 3% glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 19 (%) | |
2 | 1 | reservoir | potassium phosphate | 35 (mM) | |
3 | 1 | reservoir | glycerol | 3 (%) |