1KAC

KNOB DOMAIN FROM ADENOVIRUS SEROTYPE 12 IN COMPLEX WITH DOMAIN 1 OF ITS CELLULAR RECEPTOR CAR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	99
Spacegroup name	P 43 3 2
Unit cell lengths	167.850, 167.850, 167.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.600
R-factor	0.22
Rwork	0.220
R-free	0.25000
Structure solution method	OTHER
RMSD bond length	0.008
RMSD bond angle	1.700
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.700
High resolution limit [Å]	2.600	2.600
Rmerge	0.700	0.350
Number of reflections	24636
Completeness [%]	100.0	100
Redundancy	14.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.2		900MM AMMONIUM SULPHATE ON 100MM MES, PH 6.2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	0.9 (M)
2	1	reservoir	MES	100 (mM)	pH6.2