1KA9

Imidazole Glycerol Phosphate Synthase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	290
Detector technology	IMAGE PLATE
Collection date	2001-09-21
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	82.259, 82.259, 156.183
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.300
Rwork	0.203
R-free	0.25600
Structure solution method	MIR
RMSD bond length	0.007
RMSD bond angle	25.100 *
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.300 *	2.300 *
Rmerge	0.063	0.261 *
Total number of observations	88635 *
Number of reflections	23969 *
Completeness [%]	98.8	99.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	hepes, sodium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	sodium HEPES	10 (mM)
3	1	reservoir		4.4 (M)
4	1	reservoir	sodium HEPES	0.1 (M)	pH7.5