1K9X
Structure of Pyrococcus furiosus carboxypeptidase Apo-Yb
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-01-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0000, 1.3860 |
Spacegroup name | P 1 |
Unit cell lengths | 66.342, 86.780, 107.895 |
Unit cell angles | 88.64, 78.54, 69.44 |
Refinement procedure
Resolution | 48.780 - 2.300 |
R-factor | 0.212 |
Rwork | 0.212 |
R-free | 0.26700 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.237 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.036 | 0.087 |
Total number of observations | 353793 * | |
Number of reflections | 98094 | |
<I/σ(I)> | 26.2 | |
Completeness [%] | 93.8 | 80.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 4 * | PEG 4000, TRIS, magnesium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 19 (mg/ml) | |
2 | 1 | drop | Tris | 50 (mM) | pH8.0 |
3 | 1 | drop | glycerol | 10 (%) | |
4 | 1 | reservoir | PEG4000 | 25-30 (%) | |
5 | 1 | reservoir | Tris | 100 (mM) | pH8.5 |
6 | 1 | reservoir | 20-40 (mM) |