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1K9X

Structure of Pyrococcus furiosus carboxypeptidase Apo-Yb

Experimental procedure
Experimental methodMAD
Source typeSYNCHROTRON
Source detailsSSRL BEAMLINE BL9-2
Synchrotron siteSSRL
BeamlineBL9-2
Temperature [K]100
Detector technologyCCD
Collection date2000-01-01
DetectorADSC QUANTUM 4
Wavelength(s)1.0000, 1.3860
Spacegroup nameP 1
Unit cell lengths66.342, 86.780, 107.895
Unit cell angles88.64, 78.54, 69.44
Refinement procedure
Resolution48.780 - 2.300
R-factor0.212
Rwork0.212
R-free0.26700
Structure solution methodMAD
RMSD bond length0.007
RMSD bond angle1.237

*

Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwarePHASES
Refinement softwareCNS (1.0)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.340
High resolution limit [Å]2.3002.300
Rmerge0.0360.087
Total number of observations353793

*

Number of reflections98094
<I/σ(I)>26.2
Completeness [%]93.880.5
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP8

*

4

*

PEG 4000, TRIS, magnesium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein19 (mg/ml)
21dropTris50 (mM)pH8.0
31dropglycerol10 (%)
41reservoirPEG400025-30 (%)
51reservoirTris100 (mM)pH8.5
61reservoir20-40 (mM)

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PDB entries from 2024-09-18

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