1K9I
Complex of DC-SIGN and GlcNAc2Man3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-04-09 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9646 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.805, 148.161, 112.991 |
| Unit cell angles | 90.00, 91.03, 90.00 |
Refinement procedure
| Resolution | 26.680 - 2.500 |
| R-factor | 0.214 * |
| Rwork | 0.213 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | COMO |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.053 | 0.266 |
| Total number of observations | 250720 * | |
| Number of reflections | 59357 | |
| Completeness [%] | 97.8 | 99.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 21 * | 18% PEG 8000, 0.1M Na-cacodilate, 200mM Ca Ac2. Protein solution containing 5mM CaCl2 and 5 mM oligosaccharide (Dextra M592)., pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 5 (mM) | ||
| 3 | 1 | reservoir | PEG8000 | 18 (%) | |
| 4 | 1 | reservoir | calcium acetate | 0.2 (M) | |
| 5 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6.5 |
