1K98
AdoMet complex of MetH C-terminal fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 140 |
Detector technology | IMAGE PLATE |
Collection date | 2000-05-09 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 108.503, 108.503, 146.474 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 3.750 |
R-factor | 0.308 * |
Rwork | 0.308 |
R-free | 0.36300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1k7y |
RMSD bond length | 0.020 * |
RMSD bond angle | 1.970 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 * | 3.880 |
High resolution limit [Å] | 3.750 | 3.750 |
Rmerge | 0.062 * | 0.444 * |
Total number of observations | 34361 * | |
Number of reflections | 8057 * | |
Completeness [%] | 85.1 * | 62.8 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 22 * | cacodylate, ammonium sulfate, PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 12 (mg/ml) | |
2 | 1 | reservoir | cacodylate | 0.1 (M) | pH6.5 |
3 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
4 | 1 | reservoir | PEG8000 | 30 (%(w/v)) |