1K8R

Crystal structure of Ras-Bry2RBD complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ELLIOTT GX-18
Temperature [K]	290
Detector technology	AREA DETECTOR
Collection date	1996-08-06
Detector	SIEMENS-NICOLET
Wavelength(s)	1.54
Spacegroup name	H 3 2
Unit cell lengths	139.030, 139.030, 112.800
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 - 3.000
R-factor	0.236 *
Rwork	0.235
R-free	0.31200 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5p21
RMSD bond length	0.008 *
RMSD bond angle	1.375 *
Data reduction software	X-GEN
Data scaling software	XDS
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	3.100
High resolution limit [Å]	3.000	3.000
Rmerge	0.139	0.305 *
Total number of observations	109981 *
Number of reflections	8526	788 *
<I/σ(I)>	18.6	4.1
Completeness [%]	99.7	100 *
Redundancy	12.8	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	290	PEG 3350, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 290K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20-25 (mg/ml)
2	1	reservoir	PEG3350	20-25 (%)
3	1	reservoir	Tris-HCl	100 (mM)	pH8.
4	1	reservoir		0.2-0.4 (M)