1K8P
Structure of the Human G-quadruplex reveals a novel topology
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-05-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 56.607, 56.607, 40.546 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.400 |
| R-factor | 0.186 * |
| Rwork | 0.189 |
| R-free | 0.26100 * |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.016 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.068 | 0.280 |
| Total number of observations | 64877 * | |
| Number of reflections | 3130 * | |
| <I/σ(I)> | 15 | 9.6 |
| Completeness [%] | 99.2 * | 99.7 |
| Redundancy | 10 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 * | 285 * | MPD, KCl, LiCl, MgCl2, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 286K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | KCl | ||
| 3 | 1 | 1 | LiCl | ||
| 4 | 1 | 1 | MgCl2 | ||
| 5 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate gradient | 50 (%) | |
| 2 | 1 | drop | ammonium sulfate | 500 (mM) | |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | 50 (mM) | ||
| 5 | 1 | drop | 50 (mM) | ||
| 6 | 1 | drop | DNA | 0.5 (mM) | |
| 7 | 1 | drop | potassium cacodylate | 50 (mM) | pH6.5 |
