1K8K
Crystal Structure of Arp2/3 Complex
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-06-25 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.08 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 111.710, 130.400, 204.930 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 * - 2.010* |
R-factor | 0.216 * |
Rwork | 0.216 |
R-free | 0.24900 * |
Structure solution method | MAD |
RMSD bond length | 0.011 * |
RMSD bond angle | 1.353 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | MLPHARE |
Refinement software | REFMAC |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 * |
High resolution limit [Å] | 2.010 * |
Rmerge | 0.064 * |
Total number of observations | 852046 * |
Number of reflections | 183319 * |
<I/σ(I)> | 8.6 |
Completeness [%] | 92.8 * |
Redundancy | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 8 * | 4 * | PEG 8000, KSCN, Hepes at pH 7.5, VAPOR DIFFUSION, HANGING DROP at 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | drop | Tris | 20 (mM) | pH8.0 |
3 | 1 | drop | 100 (mM) | ||
4 | 1 | drop | EGTA | 1 (mM) | |
5 | 1 | drop | 1 (mM) | ||
6 | 1 | drop | dithiothreitol | 1 (mM) | |
7 | 1 | reservoir | PEG8000 | 6 (%) | |
8 | 1 | reservoir | KSCN | 0.1 (M) | |
9 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |