1K8G
Crystal Structure of the N-terminal domain of Oxytricha nova telomere end binding protein alpha subunit both uncomplexed and complexed with telomeric ssDNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-09-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 121.182, 121.182, 137.169 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.600 |
| R-factor | 0.246 |
| Rwork | 0.246 |
| R-free | 0.28500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | residues 37-320 of Oxytricha nova telomere end binding protein alpha subunit PDB accession 1OTC |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.557 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.103 * | 0.710 * |
| Total number of observations | 574134 * | |
| Number of reflections | 35994 | |
| <I/σ(I)> | 20.3 | 2.1 |
| Completeness [%] | 99.8 | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG4000, MES, Ammonium sulfate, DTT, Sodium Azide, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG4000 | ||
| 2 | 1 | 1 | MES | ||
| 3 | 1 | 1 | NH4SO4 | ||
| 4 | 1 | 1 | DTT | ||
| 5 | 1 | 1 | Sodium Azide |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 5 (mM) | pH7.5 |
| 10 | 1 | reservoir | 0.02 (%) | ||
| 11 | 1 | reservoir | 50 (mM) | ||
| 2 | 1 | drop | 50 (mM) | ||
| 3 | 1 | drop | dithiothreitol | 2 (mM) | |
| 4 | 1 | drop | EDTA | 0.1 (mM) | |
| 5 | 1 | drop | 0.02 (%) | ||
| 6 | 1 | reservoir | PEG4000 | 22 (%(w/v)) | |
| 7 | 1 | reservoir | MES | 50 (mM) | pH6.6 |
| 8 | 1 | reservoir | ammonium sulfate | 150 (mM) | |
| 9 | 1 | reservoir | dithiothreitol | 2 (mM) |
