1K8F

CRYSTAL STRUCTURE OF THE HUMAN C-TERMINAL CAP1-ADENYLYL CYCLASE ASSOCIATED PROTEIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	83
Detector technology	IMAGE PLATE
Collection date	2001-04-16
Detector	RIGAKU RAXIS IV
Spacegroup name	P 21 21 21
Unit cell lengths	82.640, 83.120, 99.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.800
R-factor	0.232
Rwork	0.232
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1k4z
RMSD bond length	0.005
RMSD bond angle	1.340
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.059	0.149
Number of reflections	16727
<I/σ(I)>	20.2	6.3
Completeness [%]	95.0	92.4
Redundancy	9.06	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	290	0.1M Tris-HCl, pH 8.5, 27.5% PEG4000, 0.2M NaOAc, VAPOR DIFFUSION, SITTING DROP, temperature 290K