1K7T
Crystal Structure Analysis of crosslinked-WGA3/GlcNAcbeta1,6Gal complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR571 |
Detector technology | AREA DETECTOR |
Collection date | 2000-12-29 |
Detector | ENRAF-NONIUS FAST |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.150, 93.170, 45.100 |
Unit cell angles | 90.00, 110.81, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.400 |
R-factor | 0.242 * |
Rwork | 0.242 |
R-free | 0.32600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Native WGA3 |
RMSD bond length | 0.012 |
RMSD bond angle | 24.200 * |
Data reduction software | MADNESS |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.000 | 2.450 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.116 | 0.249 |
Total number of observations | 32097 * | |
Number of reflections | 13176 * | |
Completeness [%] | 96.2 | |
Redundancy | 2.44 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 4.9 | 298 | Harata, K., (1995) Acta Crystallogr., Sect.D, 51, 1013. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | WGA3 | 2.5 (mg) | |
2 | 1 | 2 | acetate | 10 (mM) | pH4.9 |
3 | 1 | 2 | 6 (mM) | ||
4 | 1 | 2 | ethanol | 4 (%(v/v)) |