1K7Q

PrtC from Erwinia chrysanthemi: E189A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	1998-11-03
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	102.396, 102.396, 121.754
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 * - 1.800
R-factor	0.2
Rwork	0.197
R-free	0.21830 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004 *
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000 *	1.830
High resolution limit [Å]	1.800	1.800
Rmerge	0.056	0.270
Total number of observations	557664 *
Number of reflections	67917 *
<I/σ(I)>	25.9	4.9
Completeness [%]	99.5	99.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.5	20 *	NaCl, pH 6.5, VAPOR DIFFUSION, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		2 (M)
2	1	reservoir		0.1 (M)
3	1	reservoir		0.1 (M)
4	1	reservoir	MES	0.1 (M)	pH6.5