1K7K
crystal structure of RdgB- inosine triphosphate pyrophosphatase from E. coli
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-08-21 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.97937, 0.97918 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 78.180, 78.180, 80.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.090 - 1.500 |
R-factor | 0.21261 |
Rwork | 0.211 |
R-free | 0.23751 |
Structure solution method | MAD |
Data reduction software | d*TREK |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.000 | 1.500 |
High resolution limit [Å] | 1.450 | 1.450 |
Number of reflections | 40104 | |
Completeness [%] | 99.3 | 93.8 |
Redundancy | 8.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.8 | PEG4k, Am. Acetate, Na-Citrate, pH 5.8, VAPOR DIFFUSION | |
1 | VAPOR DIFFUSION | 5.8 | PEG4k, Am. Acetate, Na-Citrate, pH 5.8, VAPOR DIFFUSION |