1K7J

Structural Genomics, protein TF1

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2001-08-22
Detector	SBC-2
Wavelength(s)	0.9795, 0.9798, 0.94656
Spacegroup name	P 31 2 1
Unit cell lengths	56.260, 56.260, 135.301
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.840 - 1.400
Rwork	0.195
R-free	0.20300
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.400
Data reduction software	d*TREK
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.084	0.293
Number of reflections	49419
<I/σ(I)>	27.03	2.63
Completeness [%]	99.3	95.2
Redundancy	12.57	4.28

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	30% Ammonium sulfate, 0.1M Na citrate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K