1K7H
CRYSTAL STRUCTURE OF SHRIMP ALKALINE PHOSPHATASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-06-01 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.9310,1.2820,1.2825,0.933 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 171.169, 171.169, 84.332 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 119.500 - 1.920 |
| R-factor | 0.2 |
| Rwork | 0.198 |
| R-free | 0.22700 |
| Structure solution method | MAD |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.907 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLOMON |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.990 |
| High resolution limit [Å] | 1.850 | 1.920 |
| Rmerge | 0.076 | 0.465 |
| Total number of observations | 1131014 * | |
| Number of reflections | 103370 * | |
| <I/σ(I)> | 11.8 | 2.2 |
| Completeness [%] | 96.8 | 99.2 |
| Redundancy | 2.6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.6 | pH 5.60 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 11 (mg/ml) | |
| 2 | 1 | drop | Zn2+ | 0.1 (mM) | |
| 3 | 1 | drop | Mg2+ | 1.0 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 42.5 (%(w/v)) | |
| 5 | 1 | reservoir | glycerol | 10 (%(v/v)) | |
| 6 | 1 | reservoir | Tris-maleate | 100 (mM) | pH5.6 |
