1K7C

Rhamnogalacturonan acetylesterase with seven N-linked carbohydrate residues distributed at two N-glycosylation sites refined at 1.12 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	263
Detector technology	IMAGE PLATE
Collection date	1997-06-15
Detector	MARRESEARCH
Wavelength(s)	1.1024
Spacegroup name	P 21 21 21
Unit cell lengths	52.170, 56.920, 71.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.000 * - 1.120
R-factor	0.103 *
Rwork	0.103
R-free	0.13900 *
Structure solution method	MIR
Starting model (for MR)	1deo
RMSD bond length	0.015
RMSD bond angle	0.031
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.000	1.140
High resolution limit [Å]	1.120	1.120
Rmerge	0.060 *	0.370
Total number of observations	644629 *
Number of reflections	80624 *
Completeness [%]	97.7	82.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	Molgaard, A., (1998) Acta Crystallogr., Sect.D, 54, 1026. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		1.4 (M)	or 1.4M (NH4)2SO4
2	1	reservoir	sodium acetate	0.1 (M)