1K77
Crystal Structure of EC1530, a Putative Oxygenase from Escherichia coli
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2001-05-18 |
| Detector | SBC-2 |
| Wavelength(s) | 0.97946, 0.97957, 0.93927 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 104.907, 74.368, 39.376 |
| Unit cell angles | 90.00, 98.81, 90.00 |
Refinement procedure
| Resolution | 39.000 * - 1.630 |
| R-factor | 0.194 * |
| Rwork | 0.194 |
| R-free | 0.21400 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | 1.690 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.072 | 0.266 |
| Number of reflections | 35621 | |
| <I/σ(I)> | 7.1 | 2.7 |
| Completeness [%] | 95.7 | 70.8 |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 20 * | magnesium formate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 9 (mg/ml) | |
| 2 | 1 | reservoir | HEPES | 0.1 (M) | pH7. |
| 3 | 1 | reservoir | PEG8000 | 5 (%) | |
| 4 | 1 | reservoir | glycerol | 5 (%) |
