1K6J
Crystal structure of Nmra, a negative transcriptional regulator (Monoclinic form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-09-23 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.9537 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 148.800, 64.300, 110.200 |
Unit cell angles | 90.00, 121.80, 90.00 |
Refinement procedure
Resolution | 20.000 * - 1.800 |
R-factor | 0.164 |
Rwork | 0.165 |
R-free | 0.20200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1k6i |
RMSD bond length | 0.009 |
RMSD bond angle | 1.550 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.060 | 0.369 |
Number of reflections | 78344 | |
<I/σ(I)> | 12.8 | 1.3 |
Completeness [%] | 95.5 | 73.3 |
Redundancy | 5.2 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | Nichols, C.E., (2001) Acta Crystallogr., Sect.D, 57, 1722. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 18 (mg/ml) | |
2 | 1 | drop | ammonium sulfate | ||
3 | 1 | drop | PEG6000 | ||
4 | 1 | reservoir | 3.6 (M) | ||
5 | 1 | reservoir | Tris | 0.1 (M) | pH8.0 |