1K6I
Crystal structure of Nmra, a negative transcriptional regulator (Trigonal form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-09-23 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 76.800, 76.800, 104.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.890 - 1.800 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.25800 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.065 | 0.139 |
| Number of reflections | 29825 | |
| <I/σ(I)> | 19.4 | 9.2 |
| Completeness [%] | 88.5 | 51.6 |
| Redundancy | 10.6 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | potassium chloride, sodium dihydrogen phosphate, potassium dihydrogen phosphate, MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
