1K6F

Crystal Structure of the Collagen Triple Helix Model [(Pro-Pro-Gly)10]3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	294
Detector technology	IMAGE PLATE
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	26.907, 26.357, 182.498
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 1.300
R-factor	0.185
Rwork	0.181
R-free	0.29700
Structure solution method	ARP/WARP
RMSD bond length	0.026
RMSD bond angle	0.045
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	SHELXL-97

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000 *
High resolution limit [Å]	1.300 *
Rmerge	0.090 *
Number of reflections	23121
Completeness [%]	88.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	microgravity conditions *	5.6	294	HAc/NaAc, pH 5.6, microdialysis carried out in microgravity conditions, temperature 21K
1	microgravity conditions *	5.6	294	HAc/NaAc, pH 5.6, microdialysis carried out in microgravity conditions, temperature 21K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	polypeptide	5.0 (mg/ml)
2	1	1	acetic acid	0.05 (M)
3	1	1	sodium acetate	0.22 (M)
4	1	1	PEG400	10 (%(v/v))