1K68
Crystal Structure of the Phosphorylated Cyanobacterial Phytochrome Response Regulator RcpA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-06-23 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 76.700, 85.160, 44.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.620 - 1.900 |
| R-factor | 0.2331 |
| Rwork | 0.233 |
| R-free | 0.27700 * |
| RMSD bond length | 0.005 |
| RMSD bond angle | 22.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 * | 2.020 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.037 | 0.302 |
| Total number of observations | 301367 * | |
| Number of reflections | 22731 | |
| <I/σ(I)> | 25.2 | 3.1 |
| Completeness [%] | 96.9 | 94.6 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 4 * | sodium citrate, sodium chloride, HEPES, glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291.5K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium formate | 3.75 (M) | |
| 2 | 1 | reservoir | glycerol | 5 (%(v/v)) | |
| 3 | 1 | reservoir | 2-mercaptoethanol | 1 (mM) | |
| 4 | 1 | drop | protein | 3-5 (mg/ml) | |
| 5 | 1 | drop | 150 (mM) | ||
| 6 | 1 | drop | Tris-HCl | 10 (mM) | pH8.0 |
| 7 | 1 | drop | glycerol | 5 (%(v/v)) | |
| 8 | 1 | drop | 2-mercaptoethanol | 1 (mM) |
