1K5V
Human acidic fibroblast growth factor. 141 amino acid form with amino terminal His tag with Asn106 replaced by Gly (N106G).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-04-11 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.600, 60.120, 101.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.400 - 2.100 |
| R-factor | 0.226 * |
| Rwork | 0.226 |
| R-free | 0.30800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jqz |
| RMSD bond length | 0.008 |
| RMSD bond angle | 16.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.400 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.050 | 0.176 |
| Total number of observations | 245767 * | |
| Number of reflections | 14287 * | |
| <I/σ(I)> | 44.1 | 10.8 |
| Completeness [%] | 97.4 * | 94.6 |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 * | 298.15 | PEG20,000, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.15K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | NaPi | 50 (mM) | |
| 2 | 1 | drop | 0.1 (M) | ||
| 3 | 1 | drop | ammonium sulfate | 10 (mM) | |
| 4 | 1 | drop | EDTA | 0.5 (mM) | |
| 5 | 1 | drop | dithiothreitol | 2 (mM) | |
| 6 | 1 | reservoir | PEG20000 | 20 (%) | |
| 7 | 1 | reservoir | MES | 0.1 (M) | pH6.5 |
