1K5U

Human acidic fibroblast growth factor. 141 amino acid form with amino terminal His tag with His93 replaced by Gly (H93G).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	103
Detector technology	IMAGE PLATE
Collection date	2000-12-20
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	33.630, 97.710, 57.460
Unit cell angles	90.00, 104.30, 90.00

Refinement procedure

Resolution	32.600 - 2.000
R-factor	0.225 *
Rwork	0.219
R-free	0.30000 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jqz
RMSD bond length	0.006
RMSD bond angle	14.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.600	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.062 *	0.234
Total number of observations	102705 *
Number of reflections	21128 *
<I/σ(I)>	29.5	3.5
Completeness [%]	90.6 *	75.2 *
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	4 *	PEG 4,000, 2-propanol, magnesium chloride, hepes., pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.15K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	NaPi	50 (mM)
10	1	reservoir		0.2 (M)
11	1	reservoir	HEPES	0.1 (M)	pH7.5
2	1	drop		0.1 (M)
3	1	drop	ammonium sulfate	10 (mM)
4	1	drop	EDTA	0.5 (mM)
5	1	drop	dithiothreitol	2 (mM)
6	1	reservoir	PEG4000	20 (%)
7	1	reservoir	2-propanol	10 (%)
8	1	reservoir	HEPES	0.1 (M)	pH7.5
9	1	reservoir	PEG400	30 (%)