1K5B
Crystal Structure of Human Angiogenin Variant des(121-123)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1997-02-11 |
| Detector | XENTRONICS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 86.450, 40.410, 32.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ang |
| RMSD bond length | 0.009 |
| RMSD bond angle | 27.500 * |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.073 | |
| Total number of observations | 38216 * | |
| Number of reflections | 10158 | |
| <I/σ(I)> | 11.7 | |
| Completeness [%] | 92.3 | 90 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.2 | 16 * | sodium citrate, sodium potassium tartrate, PEG 6000, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
| 1 | Vapor diffusion * | 5.2 | 16 * | sodium citrate, sodium potassium tartrate, PEG 6000, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | in water |
| 2 | 1 | reservoir | sodium potassium tartrate | 0.2 (M) | |
| 3 | 1 | reservoir | sodium citrate | 20 (mM) | pH5.2 |
| 4 | 1 | reservoir | PEG6000 | 10-15 (%(w/v)) |
