1K5B

Crystal Structure of Human Angiogenin Variant des(121-123)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1997-02-11
Detector	XENTRONICS
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	86.450, 40.410, 32.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.800
R-factor	0.206
Rwork	0.206
R-free	0.27500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ang
RMSD bond length	0.009
RMSD bond angle	27.500 *
Data reduction software	X-GEN
Data scaling software	X-GEN
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.073
Total number of observations	38216 *
Number of reflections	10158
<I/σ(I)>	11.7
Completeness [%]	92.3	90
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.2	16 *	sodium citrate, sodium potassium tartrate, PEG 6000, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 289K
1	Vapor diffusion *	5.2	16 *	sodium citrate, sodium potassium tartrate, PEG 6000, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 289K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)	in water
2	1	reservoir	sodium potassium tartrate	0.2 (M)
3	1	reservoir	sodium citrate	20 (mM)	pH5.2
4	1	reservoir	PEG6000	10-15 (%(w/v))