1K5A
Crystal structure of human angiogenin double variant I119A/F120A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-12-20 |
| Detector | XENTRONICS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 88.120, 41.160, 33.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 * |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.28100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDN ENTRY 2ANG |
| RMSD bond length | 0.010 |
| RMSD bond angle | 27.900 * |
| Data reduction software | XDS |
| Data scaling software | X-GEN |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.087 * | |
| Total number of observations | 24206 * | |
| Number of reflections | 5235 | |
| <I/σ(I)> | 15.5 | |
| Completeness [%] | 88.0 | 32 |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.6 | 16 * | ammonium actate, sodium citrate, PEG 4000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | in water |
| 2 | 1 | reservoir | ammonium acetate | 0.2 (M) | |
| 3 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.6 |
| 4 | 1 | reservoir | PEG4000 | 30 (%(w/v)) |
