1K5A

Crystal structure of human angiogenin double variant I119A/F120A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1996-12-20
Detector	XENTRONICS
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	88.120, 41.160, 33.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.300 *
R-factor	0.193
Rwork	0.193
R-free	0.28100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDN ENTRY 2ANG
RMSD bond length	0.010
RMSD bond angle	27.900 *
Data reduction software	XDS
Data scaling software	X-GEN
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.087 *
Total number of observations	24206 *
Number of reflections	5235
<I/σ(I)>	15.5
Completeness [%]	88.0	32
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.6	16 *	ammonium actate, sodium citrate, PEG 4000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 289K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)	in water
2	1	reservoir	ammonium acetate	0.2 (M)
3	1	reservoir	sodium citrate	0.1 (M)	pH5.6
4	1	reservoir	PEG4000	30 (%(w/v))