1K4Z

C-terminal Domain of Cyclase Associated Protein

Replaces:  1F5I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9B
Synchrotron site	NSLS
Beamline	X9B
Temperature [K]	103
Detector technology	IMAGE PLATE
Collection date	1998-08-30
Detector	MARRESEARCH
Wavelength(s)	1.04
Spacegroup name	I 21 21 21
Unit cell lengths	56.520, 86.810, 160.210
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	9.990 - 2.300
R-factor	0.207 *
Rwork	0.207
R-free	0.24200
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	27.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.440
High resolution limit [Å]	2.300	2.300
Rmerge	0.038	0.100 *
Total number of observations	105428 *
Number of reflections	17479 *	1564 *
<I/σ(I)>	49.7	16.4
Completeness [%]	97.3 *	91.1 *
Redundancy	6.1	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8.5 *	16 *	PEG 4000, lithium sulfate, HEPES, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris-HCl	0.1 (M)	pH8.5
2	1	reservoir	PEG4000	27.5 (%)
3	1	reservoir	sodium acetate	0.2 (M)
4	1	drop	protein	12-24 (mg/ml)