1K4N
Structural Genomics, Protein EC4020
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-08-08 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9795, 0.9798, 0.94656 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.661, 41.783, 105.748 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 1.600 |
| R-factor | 0.21 |
| Rwork | 0.199 |
| R-free | 0.21700 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 24.400 * |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.580 |
| Rmerge | 0.049 | 0.258 |
| Number of reflections | 24275 | |
| <I/σ(I)> | 25.1 | 5.3 |
| Completeness [%] | 99.3 | 99.4 |
| Redundancy | 6.9 * | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 298 | VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG400 | 2 (%) | |
| 3 | 1 | reservoir | ammonium sulfate | 2.2 (M) | |
| 4 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
