1K4E
CRYSTAL STRUCTURE OF THE CLASS D BETA-LACTAMASES OXA-10 DETERMINED BY MAD PHASING WITH SELENOMETHIONINE
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-06-07 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9791, 0.9793, 0.9184 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.562, 102.273, 126.349 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.870 - 2.000 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.22600 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.200 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 55652 | |
| <I/σ(I)> | 7.7 | 0.6 |
| Completeness [%] | 94.1 | 75.9 |
| Redundancy | 3.6 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | ammonium sulfate, bicine, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
