1K4D

Potassium Channel KcsA-Fab complex in low concentration of K+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	200
Detector technology	CCD
Collection date	2001-04-25
Detector	BRANDEIS - B4
Wavelength(s)	1.1
Spacegroup name	I 4
Unit cell lengths	155.290, 155.290, 75.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.300
R-factor	0.218 *
Rwork	0.218
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.056 *	0.370 *
Number of reflections	39850
<I/σ(I)>	24.5	2.2
Completeness [%]	99.2	95.3
Redundancy	4.2	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.4	20 *	PEG 400,Magnesium Acetate, Sodium Acetate , pH 5.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7-15 (mg/ml)
2	1	reservoir	PEG400	20-25 (%)
3	1	reservoir	magnesium acetate	50 (mM)
4	1	reservoir	sodium acetate	50 (mM)