1K4D
Potassium Channel KcsA-Fab complex in low concentration of K+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2001-04-25 |
| Detector | BRANDEIS - B4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | I 4 |
| Unit cell lengths | 155.290, 155.290, 75.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.218 * |
| Rwork | 0.218 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.056 * | 0.370 * |
| Number of reflections | 39850 | |
| <I/σ(I)> | 24.5 | 2.2 |
| Completeness [%] | 99.2 | 95.3 |
| Redundancy | 4.2 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.4 | 20 * | PEG 400,Magnesium Acetate, Sodium Acetate , pH 5.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7-15 (mg/ml) | |
| 2 | 1 | reservoir | PEG400 | 20-25 (%) | |
| 3 | 1 | reservoir | magnesium acetate | 50 (mM) | |
| 4 | 1 | reservoir | sodium acetate | 50 (mM) |
