1K4C
Potassium Channel KcsA-Fab complex in high concentration of K+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2001-02-17 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.93 |
Spacegroup name | I 4 |
Unit cell lengths | 155.330, 155.330, 76.270 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.000 |
R-factor | 0.218 * |
Rwork | 0.218 |
R-free | 0.23300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.071 * | 0.530 * |
Number of reflections | 60798 | |
<I/σ(I)> | 22.4 | 2.3 |
Completeness [%] | 99.1 | 95.2 |
Redundancy | 3.8 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.4 | 20 * | PEG 400, Magnesium Acetate, Sodium Acetate, pH 5.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7-15 (mg/ml) | |
2 | 1 | reservoir | PEG400 | 20-25 (%) | |
3 | 1 | reservoir | magnesium acetate | 50 (mM) | |
4 | 1 | reservoir | sodium acetate | 50 (mM) |