1K4C

Potassium Channel KcsA-Fab complex in high concentration of K+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	200
Detector technology	CCD
Collection date	2001-02-17
Detector	ADSC QUANTUM 4
Wavelength(s)	0.93
Spacegroup name	I 4
Unit cell lengths	155.330, 155.330, 76.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.000
R-factor	0.218 *
Rwork	0.218
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.071 *	0.530 *
Number of reflections	60798
<I/σ(I)>	22.4	2.3
Completeness [%]	99.1	95.2
Redundancy	3.8	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.4	20 *	PEG 400, Magnesium Acetate, Sodium Acetate, pH 5.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7-15 (mg/ml)
2	1	reservoir	PEG400	20-25 (%)
3	1	reservoir	magnesium acetate	50 (mM)
4	1	reservoir	sodium acetate	50 (mM)