1K40

crystal structure of the FAT domain of focal adhesion kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X26C
Synchrotron site	NSLS
Beamline	X26C
Temperature [K]	190
Detector technology	CCD
Collection date	2001-08-08
Detector	ADSC QUANTUM 4
Wavelength(s)	1.100
Spacegroup name	C 1 2 1
Unit cell lengths	61.104, 40.163, 52.008
Unit cell angles	90.00, 103.82, 90.00

Refinement procedure

Resolution	20.000 * - 2.250
R-factor	0.224 *
Rwork	0.236
R-free	0.27500 *
RMSD bond length	0.014
RMSD bond angle	2.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	DM
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	2.250 *
Rmerge	0.048 *	0.349 *
Number of reflections	5964
Completeness [%]	98.2 *	91.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	8 *	295	PEG10k, HEPES, pH 7.0, VAPOR DIFFUSION, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	20 (mM)	pH8.0
2	1	drop		0.1 (M)
3	1	drop	dithiothreitol	1 (mM)
4	1	drop	protein	25 (mg/ml)
5	1	reservoir	HEPES	0.1 (M)	pH7.0
6	1	reservoir	PEG10000	20 (%(w/v))