1K40
crystal structure of the FAT domain of focal adhesion kinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X26C |
Synchrotron site | NSLS |
Beamline | X26C |
Temperature [K] | 190 |
Detector technology | CCD |
Collection date | 2001-08-08 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.100 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 61.104, 40.163, 52.008 |
Unit cell angles | 90.00, 103.82, 90.00 |
Refinement procedure
Resolution | 20.000 * - 2.250 |
R-factor | 0.224 * |
Rwork | 0.236 |
R-free | 0.27500 * |
RMSD bond length | 0.014 |
RMSD bond angle | 2.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | DM |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 * | |
High resolution limit [Å] | 2.250 * | |
Rmerge | 0.048 * | 0.349 * |
Number of reflections | 5964 | |
Completeness [%] | 98.2 * | 91.9 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch method * | 8 * | 295 | PEG10k, HEPES, pH 7.0, VAPOR DIFFUSION, temperature 295K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris-HCl | 20 (mM) | pH8.0 |
2 | 1 | drop | 0.1 (M) | ||
3 | 1 | drop | dithiothreitol | 1 (mM) | |
4 | 1 | drop | protein | 25 (mg/ml) | |
5 | 1 | reservoir | HEPES | 0.1 (M) | pH7.0 |
6 | 1 | reservoir | PEG10000 | 20 (%(w/v)) |