1K3O
Crystal Structure Analysis of apo Glutathione S-Transferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-06-27 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.383, 96.007, 52.889 |
| Unit cell angles | 90.00, 92.94, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.800 |
| R-factor | 0.2485 |
| Rwork | 0.246 |
| R-free | 0.33580 |
| Starting model (for MR) | 1GSE [NO WATER OR LIGAND] |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.027 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.055 | 0.292 |
| Number of reflections | 43692 | |
| Completeness [%] | 90.8 | 36.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 22% PEG3350, 0.1M Tris-Cl 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 100K |
