1K38
CRYSTAL STRUCTURE OF THE CLASS D BETA-LACTAMASE OXA-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-06-07 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 78.979, 106.899, 129.168 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.080 - 1.500 |
| Rwork | 0.198 |
| R-free | 0.21100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k4f STRUCTURE OF OXA-10 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.080 | 1.490 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Number of reflections | 86412 | |
| <I/σ(I)> | 10.4 | 2 |
| Completeness [%] | 89.4 | 50.7 |
| Redundancy | 5.4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | sodium formate, bicine, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
