1K2Y
Crystal Structure of Phosphomannomutase/Phosphoglucomutase S108A mutant from P. aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.064, 71.306, 96.586 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.750 |
R-factor | 0.1697 |
Rwork | 0.168 |
R-free | 0.19600 * |
Structure solution method | refinement of wild-type structure |
Starting model (for MR) | Native protein pdb entry 1k35 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.600 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.053 | 0.363 |
Total number of observations | 240203 * | |
Number of reflections | 49105 | |
<I/σ(I)> | 28.5 | 2.3 |
Completeness [%] | 97.4 | 98.8 |
Redundancy | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | Regni, C.A., (2000) Acta Crystallogr, D56, 761. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 12-15 (mg/ml) | |
2 | 1 | drop | MOPS | 10 (mM) | pH7.0 |
3 | 1 | reservoir | sodium potassium tartrate | 1.4 (M) | |
4 | 1 | reservoir | sodium HEPES | 100 (mM) | pH7.5 |