1K2V
E. COLI PERIPLASMIC PROTEIN FHUD COMPLEXED WITH DESFERAL
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-12-06 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 63 |
Unit cell lengths | 85.570, 85.570, 91.600 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.000 * |
R-factor | 0.22 * |
Rwork | 0.220 |
R-free | 0.24200 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1efd |
RMSD bond length | 0.702 |
RMSD bond angle | 1.098 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.040 |
High resolution limit [Å] | 2.000 * | 1.970 |
Rmerge | 0.049 | 0.205 |
Number of reflections | 22886 | |
<I/σ(I)> | 21.8 | |
Completeness [%] | 99.1 * | 97.8 * |
Redundancy | 99.1 | 97.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 298 | Na/K phosphate, HEPES, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8.3 (mg/ml) | |
2 | 1 | drop | disodium/potassium phosphate | 0.8 (M) | |
3 | 1 | drop | HEPES | 0.05 (M) | pH7.5 |
4 | 1 | reservoir | disodium/potassium phosphate | 1.6 (M) | |
5 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |